Composite

Part:BBa_K2330001:Design

Designed by: Owen Yeung   Group: iGEM17_Edinburgh_OG   (2017-10-27)


Composite brick encoding LovTAP hybrid protein under Lacl promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Illegal restriction sites removed from original coding brick allowing compatibility with additional assembly standards.

Illegal sites removed at positions 143, 231, 400 & 537.

Part was ligated into high copy plasmid pSB1C3 using the RFC10 assembly standard under lacl promoter (see BBa_K2330001)

Source

Based on Biobrick BBa_K191006 designed by Le Thanh Tu NGUYEN (Group: iGEM09_EPF-Lausanne 2009)

Biobrick was synthesised by IDT Integrated DNA Technologies

References

Strickland, D., Moffat, K. and Sosnick, T. (2008). Light-activated DNA binding in a designed allosteric protein. Proceedings of the National Academy of Sciences, 105(31), pp.10709-10714.